S. Tohidi, M. Aghaie-Khafri* and M. Kadivar Pages 1 - 15 ( 15 )
Background: In recent years, the field of biomedical engineering, particularly drug delivery, has seen significant advancements in the evaluation of various drug carriers. Metal-organic frameworks (MOFs), are porous materials with strong coordination bonds. They have emerged as a promising tool for enhancing the effectiveness of drug delivery. Objective: In this investigation, the effect of chitosan coating on cyclophosphamide loading of MIL-100(Fe) was studied in computational and experimental way. Methods: The chitosan-coated MIL-100(Fe) containing cyclophosphamide (MIL-100(Fe)/CS/CP) was characterized by SEM, FTIR, BET, DLS, and powder X-ray diffraction analysis. The drug loading and release process were quantified using UV-spectroscopy. In vivo-In vitro study was performed. Results: The result of drug loading in chitosan-coated MIL-100(Fe) with a drug payload of 32% revealed a significant increase compared with the MIL-100(Fe) with a 26.41% payload. According to the DLS analysis the existence of chitosan increase MIL-100(Fe) particle size (381 to 463nm) and change the zeta potential from 18 to -17mV. The toxic effect of MIL-100(Fe)/CS/CP was determined on human breast cancer (MCF-7) cells. In vivo images and H&E analysis shows inhibition properties of MIL-100(Fe)/CS/CP on tumor cells. The amount of drug loading of MIL-100(Fe) particles and MIL-100(Fe)/CS was simulated using molecular dynamic software LAAMPS. Conclusion: MIL-100(Fe) was synthesized biofriendly at room pressure and temperature with an Iron (II) chloride source coated with chitosan (CS) a natural polysaccharide. Incorporating MIL-100(Fe) with this natural polymer enhanced the drug loading capacity of MIL-100(Fe) and controlled drug release.
MIL-100(Fe), chitosan, anticancer drug carrier, molecular dynamic simulation (LAAMPS).